Activity of essential oils from Syzygium aromaticum and Rosmarinus officinalis against growth and ochratoxin A production by Aspergillus tubingensis and Aspergillus luchuensis from Moroccan grapes

Essential oils have been used since ancient times in traditional medicine and agri-food science to preserve food, combat human diseases. (EOs) from clove rosemary, obtained by hydro-distillation, were analyzed GC/MS evaluated for their antifungal activity against strains of Aspergillus tubingensis A. luchuensis effect on ochratoxin A (OTA) production these fungi. The major constituent EO was eugenol (86.4%), rosemary eucalyptol (35.9%). Mycelium growth inhibition assays showed that the EOs had dose-depend effects, which reached 36.6% EO, 100% at concentration 200 μL L-1. These reductions different strains, essential oil type, strain/oil type interaction. Analyses OTA culture medium extracts carried out using HPLC-FLD, confirmed LC-MS/MS positive controls two OTA-producing tubingensis. In general, reduced (from 45 reduction), except tubingensis, where stimulated.